Why Retham™ Cryoplatelets?
Traditional platelet sourcing presents key challenges:
- Logistical burden of donor recruitment and blood collection
- High costs without in-house donor programs
- Donor variability impacting assay reproducibility
Retham™ Cryoplatelets address these limitations by providing a ready-to-use, standardized platelet source.
Product Features
- Human platelets derived from healthy donors
- Blood group O pooled platelets
- Three-donor pools per lot for reduced variability
- Cryopreserved for on-demand use
- Compatible with a wide range of functional platelet assays
Functional Performance
General workflow:
- Thaw at 37°C (~3 minutes)
- Remove cryoprotectant with centrifugation
- Resuspend in an appropriate activation buffer/normal plasma
- Stimulate with agonists
- Measure functional readouts
Granule Release Assays
Retham™ Cryoplatelets demonstrate functional responses in granule release formats, including:
- Responsiveness to TRAP (Thrombin receptor activating peptide)
- Intact FcGRIIa (CD32)-mediated signaling as evidenced by antibody-mediated activation
Aggregation-Based Assays
Demonstrated responsiveness to multiple agonists:
- TRAP
- Collagen
- Ristocetin
- ADP
- Epinephrine
- Arachidonic Acid
While higher agonist concentrations may be required versus fresh platelets, Retham™ Cryoplatelets offer broad agonist responsiveness, unlike many lyophilized alternatives.
Procoagulant Activity
Retham™ Cryoplatelets support robust coagulation-based assays:
- Thrombin Generation (CAT): ~2x higher thrombin generation vs. fresh platelets
- Thromboelastography: Strong clot formation
- Shortened lag phase observed across assays
These characteristics suggest a procoagulant phenotype, potentially reflecting partial activation or altered membrane dynamics post-cryopreservation.
Applications
Retham™ Cryoplatelets are ideal for:
- Functional platelet assays
- Drug discovery and screening (including antibody therapeutics)
- Assay development and validation
- Standardization of platelet-based workflows
- Hemostasis and thrombosis research
Benefits to Your Lab
- Eliminate donor dependency
- Improve reproducibility with standardized platelet lots
- Scale experiments efficiently
- Reduce operational complexity and cost
- Integrate seamlessly into existing workflows
Distribution
Prolytix is the authorized distributor of Retham™ Cryoplatelets, providing access, support, and technical expertise to help integrate this solution into your research programs.
For ordering information, technical support, or product inquiries, please contact Prolytix.
Important Information
- Research Use Only (RUO) – Not for diagnostic or therapeutic use
- Human-derived material – Handle using appropriate laboratory safety procedures

