N-Glycan Analysis

N-Glycan and Glycopeptide Analysis

Have confidence in the consistency of your biotherapeutics manufacturing with glycosylation, including N-glycan analysis, from Prolytix. Our expertise includes tailored methods for neutral and highly sialylated glycans, mass spectrometry detection for glycan identity, and fluorescence detection for routine use.

Glycosylation, a common post-translational modification (PTM), plays an important role in recombinant protein bioactivity and pharmacokinetics (PK). Glycosylation is also an indicator of manufacturing consistency. We provide glycopeptide map as well as released N-glycan profiling data to give you the information you need for consistent, quality development.

N-Glycan Analysis Use Cases

  • Glycopeptide mapping
    • We can characterize glycans early in product development — as early as the cell line selection stage — to help clients pick a clone that produces product with the desired glycosylation profile.
    • We can analyze process intermediate samples to ensure that fraction pooling maximized the yield of a high-quality product, which is especially important when glycosylation is critical to potency or PK properties.
  • Released N-glycan profiling: We use released N-glycan profiling with fluorescence and/or HRMS detection to look at the consistency of N-glycosylation in purified proteins.

Advanced Analytical Techniques

Prolytix uses liquid chromatography (LC) with tandem high-resolution mass spectrometry (HRMS) and fluorescence detection to analyze some of the most complex glycoproteins found in nature. Our experience includes characterization of products from cell culture, plasma, and milk.

Shopping Cart