Why Choose Retham™ HIT Antibody?
Reproducible Performance
- Monoclonal antibody design minimizes lot-to-lot variability
- Ensures consistency across experiments and laboratories
Dual-Use Across HIT Assays
- Compatible with both:
- HIT antigen assays (PF4/heparin ELISA, CLIA, LIA)
- Functional assays (e.g., serotonin release assay, SRA)
Functionally Active HIT Antibody
- Induces platelet activation in functional assays
- Mimics clinically relevant HIT antibody behavior
Broad Platform Compatibility
- Validated across multiple HIT testing platforms
- Supports cross-platform standardization
Quality Control Ready
- Ideal for:
- Assay validation
- Routine quality control (QC)
- Proficiency testing programs
Performance Highlights
HIT Antigen Assays
- Positive in 6 of 7 major HIT immunoassays
- Demonstrates heparin-dependent inhibition, consistent with true HIT antibodies
Functional HIT Assays (SRA)
- Strong platelet activation at low heparin concentrations
- Inhibition observed at high heparin concentrations
- Accurately replicates pathogenic HIT antibody behavior
Recommended Use
Research Use Only (RUO) – Not for diagnostic or therapeutic use
Suggested Starting Concentrations:
- Functional assays (SRA): 25 µg/mL
- Antigen assays: 10 µg/mL
Note: Optimization is recommended based on specific assay conditions.
Applications
Retham™ HIT Antibody is ideal for:
- HIT assay development
- Assay validation and verification
- Quality control programs
- Proficiency testing
- Cross-platform assay standardization
- Platelet activation studies