Sequence Variant Analysis Services for Therapeutic Proteins
Don’t let sequence variants halt your viral vector or recombinant protein product development. Sequence variant analysis (SVA) is a challenging discipline that involves careful data analysis. Undetected sequence variants can affect a product’s safety and efficacy. Because sequence variants affect biological properties, early detection is key.
Prolytix has extensive experience with variant projects in microbial and mammalian expression systems. We’ve seen variants that range from ppm levels up to 10% at a particular site.
Sequence Variant Analysis Services Using High-Resolution Mass Spectrometry
Prolytix designs SVA experiments to maximize coverage of proteins at the MS/MS fragment ion level. Using high-resolution mass spectrometry (HRMS), we scour the data for all possible single-base variants and manually confirm the search engine results to ensure absolute accuracy. For each sequence variant, we report the %SV (or ppm) relative to the native/wild-type peptide.
When you partner with Prolytix, you partner with an analytical and bioanalytical CRO that’s dedicated to the purity of your product. You’ll get the information you need, when you need it, to move your project forward.